9 standard spot numbers, Standard spot numbers -32 – Bio-Rad EXQuest Spot Cutter User Manual

Page 223

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PDQuest User Guide

6-32

With your higher level MatchSet open, select Transfer MrpIs from the Analyze >
MrpI Standards submenu. In the first pop-up box, select the MatchSet member you
want to copy from; in the second box, select the member you want to copy to. You
will be asked to confirm the transfer.

Repeat the procedure to apply the values to all the higher level MatchSet members.

6.9

Standard Spot Numbers

Standard spot (SSP) numbers are unique numbers assigned to each spot in the
MatchSet Master. In general, spots in the lower left region of the image have low SSP
numbers, while spots in the upper right region have high SSP numbers.

Note:

SSP numbers are increasingly important when you begin comparing spots
between different MatchSets using higher level MatchSets.

SSP numbers are automatically assigned when you create the MatchSet Master. To
create SSP numbers, PDQuest first divides the Master into rows and columns, placed
so that there are approximately the same number of spots in each section and that no
section has over 100 spots. The first two digits of a spot’s SSP number correspond to
its section’s X and Y coordinates. Spots within a section are numbered sequentially.

Displaying SSP Numbers

The spot number tools are located on the Analyze > Spot Numbers submenu.

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