2 how to use background subtraction, Warning – INFICON HAPSITE Smart Plus Chemical Identification System User Manual

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HAPSITE Smart Plus Operating Manual

9.5.2 How to Use Background Subtraction

WARNING

Be careful not to subtract a data/peaks that may be
needed for proper identification within the
chromatogram.

Background subtraction is a key component in "cleaning up" spectra before

attempting to search against a database such as NIST. In order to obtain
background spectra, there are two triangles ("cursors"), one with one blue line
designated as Background 1 (B1), the other with two blue lines designated as
Background 2 (B2), located at the bottom left of the TIC window.

NOTE: All background subtractions are indicated in the Spectrum window by

the designation Scan Number - B1(range) - B2(range).

9.5.2.1 Steps Required to Perform a One Point Background Subtraction

Figure 9-10 Original Chromatogram

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