INFICON HAPSITE Smart Plus Chemical Identification System User Manual

Page 433

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HAPSITE Smart Plus Operating Manual

Duplicate Row. . . . . . . . . . . . . . Creates a duplicate entry for the highlighted

row.

Fill down . . . . . . . . . . . . . . . . . . Replaces the contents of all rows below the

highlighted row with the name of the selected
compound.

Clear All . . . . . . . . . . . . . . . . . . . Erases all entries in the Template.

Clear All Empty

Compound Entries . . . . . . . . . . Deletes all entries that do not have a

compound name associated with them.

NOTE: The down arrow next to the compound name can be used to select a

different name if more than one possible match has been determined
by the AMDIS search.

10a

For this file, all of the blank entries are to be deleted. Click the Right Mouse
Button in the Compound field and select Clear All Empty Compound
Entries
. Refer to

Figure 12-13

.

HINT: If a compound is unidentified (blank), but is known to the analyst, name

the peak by highlighting the blank compound name field and entering
the name.

10b

Next, the Internal Standards must be selected. When using Internal Standards,
best practice is to use a quant ion from the internal standard that is close in
mass to the quant ion of the compound to be quantitated. The software always
selects the largest mass fragment in the spectrum as the quant ion. To change
the quant ion, highlight the field and type in the new number. Change the quant
ion for TRIS from 213 to 75. The software will warn that a re-calibration is
required. This is true if a quant ion is changed after calibration. To signify which
quant mass is being used, change the name of TRIS HAPSITE IS #1 to
TRIS_75. See

Figure 12-15

.

NOTE: If the name of the analyte begins with TRIS or BPFB the software will

recognize the analyte as a HAPSITE IS and automatically enter the
concentration from the IS canister into the method for calibration and
quantitation.

10c

More than one quant ion can be used from a single Internal Standard peak.
Highlight the second Internal Standard and click the Right Mouse Button, select
Duplicate Row, then change the name of the Internal Standard peaks to
BPFB_79 and BPFB_117. Next the Quant Ion should be changed to 79 and
117
. See

Figure 12-15

.

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